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GenTarget
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iCell Bioscience Inc
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Biomol GmbH
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Federation of European Neuroscience Societies
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Shanghai Huayi Company Ltd
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Image Search Results
Journal: PLoS ONE
Article Title: Bioactivity of a modified human Glucagon-like peptide-1
doi: 10.1371/journal.pone.0171601
Figure Lengend Snippet: Mouse pancreatic β cell proliferation (A), RT-qPCR analysis of gene expression (B), and insulin secretion (C) . MIN6 cells were seeded into flat-bottomed 96-well microtiter plates at a density of 6.0×10 4 cells per well. ( A ) Cell proliferation assay: cells were treated with various concentrations (0.3, 3, 15 and 30 μM) of mGLP-1 or GLP-1 for 48h, 10 μL CCK8 was then added to each well and incubated for 1 to 4 hours to determine the optimal reading of OD 450 . The control was cell cultures treated with DPBS only. ( B ) Relative expression of genes related with proliferation and apoptosis in cells treated with mGLP-1, GLP-1, or DPBS as determined by RT-qPCR (n = 3). ( C ) Insulin secretion assay: Mouse pancreatic β cells were incubated with 15 μM GLP-1 or mGLP-1 in the presence of glucose (10 mM) for 30, 60, 90, or 120 min. The controls were cells incubated with just DPBS in the presence of 10mM glucose. Cell culture supernatants were collected and insulin concentration determined using a mouse insulin ELISA kit (JiNingShiYe Ltd. Shanghai, China). Data shown represent the mean ±SD (n = 6). Different letters or (*) indicate significant difference at p <0.05.
Article Snippet:
Techniques: Quantitative RT-PCR, Gene Expression, Proliferation Assay, Incubation, Control, Expressing, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay
Journal: PLoS ONE
Article Title: Bioactivity of a modified human Glucagon-like peptide-1
doi: 10.1371/journal.pone.0171601
Figure Lengend Snippet: Cell viability ratio (CRV) of MIN6 cells treated with different concentrations of GLP-1 or mGLP-1.
Article Snippet:
Techniques: